Birthdating studies

Investigators took advantage of this property and designed a clever pulse-chase experiment to investigate whether different cell types exited the cell cycle at characteristic times.

Animals at various stages of development were given a pulse of 3[H]-thymidine (3Hd T).

An interesting property of retinal cells is that they do not continue to divide after they differentiate.

This means that at some point in development, a progenitor permanently exits the cell cycle, referred to as its birthdate.

Cells that retained maximum labeling are those that exited the cell cycle (born) on the day of 3Hd T administration.

More recently, birthdating studies have been conducted with synthetic nucleotides, such as 5-bromo-2-deoxyuridine (Brd U), which can be detected by antibodies instead of autoradiography.

contributed significantly to the experimental and technical design.

The intermediate and anterior lobes of the pituitary gland are derived from an invagination of oral ectoderm that forms Rathke's pouch.

During gestation proliferating cells are enriched around the pouch lumen, and they appear to delaminate as they exit the cell cycle and differentiate.

contributed significantly to generation of the data.

contributed significantly to supervision and management of the project.

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We conducted a birth dating study of Rathke's pouch derivatives to determine whether the location of specialized cells at birth is correlated with the timing of cell cycle exit.

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